                         SEQUENCE LISTING

<110>  TransAlgae Ltd
       Gressel, Jonathan
 
<120>  Decreasing RUBISCO content of algae and cyanobacteria cultivated 
       in high carbon dioxide

<130>  Transalgae -Rubisco

<150>  61/191,169
<151>  2008-09-05

<150>  62/292,453
<151>  2008-09-09

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  774
<212>  DNA
<213>  artificial

<220>
<223>  chemically synthetized


<220>
<221>  misc_feature
<222>  (1)..(774)
<223>  RNAi cassette for Chlamydomonas reinhardtii RbcS2 gene, encodes 
       for ribulose-1,5-bisphosphate carboxylase/oxygenase (RUBISCO) 
       small subunit 2:

<400>  1
ggatcctcta gagtcactca acatcttaaa atggccgccg tcattgccaa gtcctccgtc       60

tccgcggccg tggcccgccc ggcccgctcc agcgtgcgcc ccatggccgc gctgaagccc      120

gccgtcaagg ccgcccccgt ggctgccccg gctcaggcca accagatgat ggtctggacc      180

ccggtcaaca acaagatgtt cgagaccttc tcctacctgc cccccctgag cgacgagcag      240

atcgccgccc aggtcgacta cattgtaagt ctggcgagag cccgacgggt ccactgtggc      300

actgggttag cttttggcac acgggtccac tgtggcactg gttagcttgg caccgggaca      360

gcgcctatct caccgcgggg aactgacgca tacccctgct cgtgcttcag cacggaaaag      420

caaggggccc aattccatct ttggtggttc tgtgcgctgg tgactgaacc tcttctccct      480

cccatttccc gtgcgcccgc agctgtacta aatgtagtcg acctgggcgg cgatctgctc      540

gtcgctcagg gggggcaggt aggagaaggt ctcgaacatc ttgttgttga ccggggtcca      600

gaccatcatc tggttggcct gagccggggc agccacgggg gcggccttga cggcgggctt      660

cagcgcggcc atggggcgca cgctggagcg ggccgggcgg gccacggccg cggagacgga      720

ggacttggca atgacggcgg ccattttaag atgttgagtg actctagagg atcc            774


<210>  2
<211>  29
<212>  DNA
<213>  artificial

<220>
<223>  chemically synthetized


<220>
<221>  misc_feature
<222>  (1)..(29)
<223>  primer BstBI-rbcS2B

<400>  2
gcttcgaatc aacgagcgcc tccatttac                                         29


<210>  3
<211>  28
<212>  DNA
<213>  artificial

<220>
<223>  chemically synthetized


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  primer Xho1-rbsS2 AS

<400>  3
gcctcgagat ggccgccgtc attgccaa                                          28


<210>  4
<211>  375
<212>  DNA
<213>  Pichia pastoris


<220>
<221>  misc_feature
<222>  (1)..(375)
<223>  bleomycin binding protein(Ble) gene sequence

<400>  4
atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc       60

gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt      120

gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac      180

aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag      240

gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag      300

ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc      360

gaggagcagg actga                                                       375


<210>  5
<211>  822
<212>  DNA
<213>  Chalmydomonas sp.


<220>
<221>  promoter
<222>  (1)..(822)
<223>  Chlamydomonas psaD promoter

<400>  5
gcggccgcca cacacctgcc cgtctgcctg acaggaagtg aacgcatgtc gagggaggcc       60

tcaccaatcg tcacacgagc cctcgtcaga aacacgtctc cgccacgctc tccctctcac      120

ggccgacccc gcagcccttt tgccctttcc taggccaccg acaggaccca ggcgctctca      180

gcatgcctca acaacccgta ctcgtgccag cggtgccctt gtgctggtga tcgcttggaa      240

gcgcatgcga agacgaaggg gcggagcagg cggcctggct gttcgaaggg ctcgccgcca      300

gttcgggtgc ctttctccac gcgcgcctcc acacctaccg atgcgtgaag gcaggcaaat      360

gctcatgttt gcccgaactc ggagtcctta aaaagccgct tcttgtcgtc gttccgagac      420

atgttagcag atcgcagtgc cacctttcct gacgcgctcg gccccatatt cggacgcaat      480

tgtcatttgt agcacaattg gagcaaatct ggcgaggcag taggctttta agttgcaagg      540

cgagagagca aagtgggacg cggcgtgatt attggtattt acgcgacggc ccggcgcgtt      600

agcggccctt cccccaggcc agggacgatt atgtatcaat attgttgcgt tcgggcactc      660

gtgcgagggc tcctgcgggc tggggagggg gatctgggaa ttggaggtac gaccgagatg      720

gcttgctcgg ggggaggttt cctcgccgag caagccaggg ttaggtgttg cgctcttgac      780

tcgttgtgca ttctaggacc ccactgctac tcacaacaag cc                         822


<210>  6
<211>  3001
<212>  DNA
<213>  artificial

<220>
<223>  chemically synthetized


<220>
<221>  misc_feature
<222>  (1)..(3001)
<223>  Synechococcus PCC7002 rbcL region

<400>  6
tttcaacgag ggcaggcatg tgccatacgt ggataccacc ggaagccaca ggcatggtgc       60

cggggagaga agcgtagtct tgggtgaaga atacaccgcg agaacgatct tcttcaacgt      120

agtcttcacg catcaggtct acgaaaccga gggtggcggc gcgatcgcct tcgagcttac      180

caacaaccgt accggagtgg aggtggtcac caccagagag gcggagacac ttagcgagaa      240

cgcggaagtg aataccgtgg ttcttctgac ggtcgattac cgcgtgcatt gcccggtgga      300

tgtggagcag aacgccgtta tcacgacacc acttcgcaag ggtagtattc gcagtgaaac      360

caccagttaa gaagtcgtgc atgatgatgg gagtgccgat ttccttagcg aattcagccc      420

gcttgagcat ttcttcgcaa gtgccagcgg tgacgttaag gtagtgaccc ttaacttcgt      480

tggtttcagc ttgggatttt tcgatagctt cttgaacgaa caggaagcga tcgcgccaac      540

gcatgaaagg ctgagagttg atgttttcgt catctttggt gaagtcaaga ccaccacgga      600

gacattcata aaccgcacga ccgtagttct tcgcagacag accgagcttc ggcttaatcg      660

tacaaccgag gagaggacga ccatacttgt tgaggaggtc acgctctaca gtgatcccgt      720

ggggaggccc ttggtaagtt ttgattaacg caacggggaa gcggatatct tcgaggcgca      780

gggcacgcag cgctttaaaa ccgaatacgt taccaaccaa ggaagtcaaa acgttggtta      840

cagaaccttc ttcaaacaga tcgagggggt aagcaacgaa acagaaatat tggttgtctt      900

caccgggaac gggttcaaca ttgtagcaac gacccttgta gcggtcgagg tcagttaaac      960

catcggtcca tacagtggtc caagtaccgg tagaagattc agccgcaaca gccgcagcac     1020

attcttcggg ggggactcca ggttggggag tcatccggaa acaagcgagt aagtcggtat     1080

ctttcggggt gtaatcgggg gtgtagtaag tcaggcggta gtcctgtaca ccggcattaa     1140

acccagcaga tttggtctga accatgcggt tttcctccag caaaaatgct tatctttaac     1200

agacaaatac cagtaacggt attgttggtc gaaaacttca aaaatcttac gttggcaaaa     1260

ctgcttttta aaattctgaa gattcaagtc ttatgacttt ctttaatctg tgggatatgt     1320

taccacagcc tcgacttatt tttatctttt agcaacaaaa aaagattgct tttgtttttt     1380

gggctgatta gcatttctaa tgctgtttga tgggcctatt ttacccttac aaaaatacct     1440

aaaaaagcca taaaatcccg ctcgaattca gccatgctac ctaatgagac attgggctaa     1500

aaccgttttc tgtcgggatc tttagagagg gaaagcgttc aaatgagatg aattaagtat     1560

ttatgtttct caacaatctc ctttaagaac tttaaacatt taattttcac tcaagcaatc     1620

atgaaagttt tgtggggcta aggctcatct ggttgatttg gggtattgag agaattttgg     1680

tgagggaata gggtcattaa tagttgattg atataaactt gcccgacaac tggtgttttt     1740

tcgcccgatt ctagggattc ctttgattct ggaaccgtcg gcatcacttc ggtgtttggt     1800

gtttcagttg gaggggatcc tgcttctggt gggggctgga cattgggacg attgctgctg     1860

ctagtgggcg atcgccctgg tggcgaaaag gttgtggcag tctggttagt tgtatcttct     1920

gcctgccaag gatcgagggg ttgggttgtc gattgattcg cgggttttgg cggttgagtc     1980

tggtaagaag cggtggtttc ttggcgggac ggatcgccga tgaggctttg gggcgggaga     2040

attgcggcgg cggcgatcgc cgcttgaaat agggtagagc cataacctaa gcaggcattt     2100

tctcccactg agcattggcc aatgatcagg cagcctgccc cgataatcgc gccggcatgg     2160

atctcgatgt tgccgccgtg ggcctggata atcgcacctg caccaataca agcaccagtg     2220

gcgatcgcca cataattgcc ttcggttgct tgcagaataa caccgggggc aataaccgca     2280

cggggatgaa tccttacatc gccactaatt tgaatatctg gatgggtaat tgcctggaaa     2340

gtcacaatga ttctgtcaac ggtgattcaa ttaatcacag gtggggtaag ggttggggga     2400

tagacattcg ttttctcccc cataacgaag tggtcgcgtt acacaaccta ggggcgttgg     2460

ataatttcct caagcacccg tcgtttagcc gcttcatcga caccaatcat gcggacatat     2520

tcgccactgt gctcctggag acaaccttcg aggtgacgta aaacttctgc ctcttgggtg     2580

ctgtcaatgg tgccacaact actccaggac ttaacacgga aacggcgctt atcggcgtgt     2640

tctgttccaa tcttgtagcc ttgcatcaaa agcgaacgga ctttagaaac cacatcccca     2700

ctgagactcc cgctactgtg acccccgaaa ccattgctgc ttggggctgc tttcgtgcct     2760

cccgaaaaac tactggttgc ggttgctgtc tggacaggcg cattgttgcc agggcgttgg     2820

atgatgatct ctgcggcccg tgttttggag ttggggtcaa cggcgattaa ctggacatat     2880

tcgttgggga actgggcggc gatcgcctgg atatttgcca aaatctgatt agcagaatga     2940

ccctccacaa agcctgcccc tagccaagac tgggttttaa aacggcgagt gctggcgtgt     3000

t                                                                     3001


<210>  7
<211>  792
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(792)
<223>  aadA gene sequence

<400>  7
atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc       60

gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc      120

ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa      180

acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc      240

gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt      300

tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt      360

atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa      420

catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag      480

gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct      540

ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc      600

agaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat      660

cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc      720

tcgcgcgcag atcagttgga agaatttgtt cactacgtga aaggcgagat caccaaggta      780

gtcggcaaat aa                                                          792


<210>  8
<211>  1935
<212>  DNA
<213>  artificial

<220>
<223>  chemically synthetized


<220>
<221>  misc_feature
<222>  (1)..(1836)
<223>  FBP/SBPase-3HA gene sequence (From Synechococcus PCC       7942, synthesized according to Chlamydomonas r. codon usage

<220>
<221>  misc_feature
<222>  (1837)..(1935)
<223>  3 x HA tag

<400>  8
atggccgccg tcattgccaa gtcctccgtc tccgcggccg tggcccgccc ggcccgctcc       60

agcgtgcgcc ccatggccgc gctgaagccc gccgtcaagg ccgcccccgt ggctgccccg      120

gctcaggcca accagatgga gaagactatt ggcctggaga tcattgaggt ggtggagcag      180

gccgcgatcg cctccgctcg cctcatgggc aagggcgaga agaacgaggc tgaccgcgtg      240

gccgtggagg cgatgcgggt gcgcatgaac caggtggaga tgctgggccg catcgtgatt      300

ggcgagggcg agcgcgacga ggcgcccatg ctgtatatcg gcgaggaggt gggcatctac      360

cgcgacgcgg acaagcgcgc gggtgtgccc gccggcaagc tggtggagat cgacattgcc      420

gtggacccct gcgagggcac caacctgtgc gcgtacggcc agccggggtc catggccgtc      480

ctggccatca gcgagaaggg cggcctgttc gcggcccccg acttctacat gaagaagctg      540

gcggctcctc cggcggcgaa gggcaaggtc gacattaaca agtcggccac ggagaacctg      600

aagatcctgt ccgagtgcct ggaccgggcc atcgatgagc tggtggtggt cgtgatggac      660

atggccgccg tcattgccaa gtcctccgtc tccgcggccg tggcccgccc ggcccgctcc      720

agcgtgcgcc ccatggccgc gctgaagccc gccgtcaagg ccgcccccgt ggctgccccg      780

gctcaggcca accagatgga gaagactatt ggcctggaga tcattgaggt ggtggagcag      840

gccgcgatcg cctccgctcg cctcatgggc aagggcgaga agaacgaggc tgaccgcgtg      900

gccgtggagg cgatgcgggt gcgcatgaac caggtggaga tgctgggccg catcgtgatt      960

ggcgagggcg agcgcgacga ggcgcccatg ctgtatatcg gcgaggaggt gggcatctac     1020

cgcgacgcgg acaagcgcgc gggtgtgccc gccggcaagc tggtggagat cgacattgcc     1080

gtggacccct gcgagggcac caacctgtgc gcgtacggcc agccggggtc catggccgtc     1140

ctggccatca gcgagaaggg cggcctgttc gcggcccccg acttctacat gaagaagctg     1200

gcggctcctc cggcggcgaa gggcaaggtc gacattaaca agtcggccac ggagaacctg     1260

aagatcctgt ccgagtgcct ggaccgggcc atcgatgagc tggtggtggt cgtgatggac     1320

cggccgcgcc acaaggagct catccaagag atccgccagg cgggtgcccg ggtgcgcctg     1380

atcagcgacg gggacgtgag cgcggctatc agctgcggct tcgcggggac caacacccac     1440

gccctgatgg gcatcggcgc cgctcctgag ggcgtgatta gcgccgcggc gatgcgctgc     1500

ctgggcggcc actttcaggg ccagctgatc tacgacccgg aggtggtgaa gacgggcctc     1560

atcggcgagt cgcgcgagtc gaacatcgcc cggctgcagg agatggggat cacggacccc     1620

gaccgcgtgt acgatgctaa cgagctggct tcgggccagg aggtcctctt cgccgcctgc     1680

ggcatcaccc ccggcctgct gatggagggc gtccgcttct tcaagggtgg cgcccggacc     1740

cagagcctcg tcatttcctc gcagtcgcgc acggcccgct tcgtggacac cgtccacatg     1800

ttcgacgacg tgaagaccgt gagcctgcgc ctggagtacc cctacgacgt gccggactac     1860

gcgggctacc cttacgacgt ccccgattat gccggttcct acccgtacga tgtgcccgac     1920

tacgccgccc agtaa                                                      1935


